【文獻標(biāo)題】Effects of phoeriod on morphology ａnd function in testis ａnd epididymis of Cricetulus barabensis

【作者】Junjie Mou，Jinhui Xu，Zhe Wang，et.al

【作者單位】曲阜師范大學(xué)（Qufu Normal University）

【文獻中引用產(chǎn)品】

倉鼠褪黑素（MT）elisa試劑盒

倉鼠促卵泡生長激素（FSH）ELISA試劑盒

倉鼠促黃體生成素（LH）ELISA試劑盒

倉鼠睪酮（T）ELISA試劑盒

【關(guān)鍵詞】apoptosis，auhagy，Cricetulus barabensis，mitochondria，phoeriod

【DOI】10.1002/jcp.29998

【影響因子(IF)】5.20

【出版期刊】《journal of cellular physiology》

【產(chǎn)品原文引用】

At the end of the exposure, the animals were killed by CO2 asphyxiation at 22:00 on the final day after all of the hamsters had been in the dark for at least 2 hr (Z. Wang, Xu, Mou, Kong, Wu,et al., 2020). Blood samples were immediately collected after sacrifice ａnd stored at 4°C for 30 min, then centrifuged at 3,000 rpm for 15 min at 4°C. Serum MT, FSH, LH, ａnd fecal TTE levels were estimated using an enzyme‐linked immunosorbent assay Labsystems Multiskan MS 352 (Shanghai Hengyuan Biological Technology Co.,Ltd., H‐40277, Shanghai, China). Testis ａnd epididymis were removed ａnd weighed. The right testis or epididymis was cut into three parts;one part was immersed in paraformaldehyde‐glutaraldehyde fixative for transmission electron microscopy (TEM), while the other two parts were immersed in 4% paraformaldehyde for paraffin ａnd frozen section embedding. The left testis or epididymis was stored in a refrigerator at 80°C for subsequent western blot experiments.

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原創(chuàng)作者：上海恒遠生物科技有限公司